Reporter

Part:BBa_K4161605:Design

Designed by: Zhujun Yao 姚竹君   Group: iGEM22_DKU   (2022-09-27)


Flag-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Flag tag should be used on both protein pull-down and detection. It should be different from the tag in ipaD expressed bacteria.


Source

Flag tag is firstly used by Munro and Pelham, in 1984.

References